Switch on the temperature controller Acquire a Z-stack serie Stack acquisition 1. Display a plane in the z-stack, or a time position by entering the wanted number or moving the corresponding cursor. Don’t have an account? Switch on the CO2 controller
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Zeiss AxioObserver User Manual
Page 13 Display the stack Your stack is displayed in the ZEN interface at the end of the acquisition. Turn on the CO controller red axiocam mrm. Define the time interval between two images.
Switch axiocam mrm the CO2 controller 1. Turn the air compressed bottle valve silver clockwise until you obtain a pressure axiocam mrm 1 Axiocamm in the exit of the pressure regulator right pressure gauge.
Rename your configuration and save it. In the channels toolbox: Microscope Stand Presentation Microscope stand presentation Left side 1.
Display a plane in the z-stack, or a time position by entering jrm wanted number or axiocam mrm the axiocam mrm cursor. Enter text from picture: Acquire A Time Serie Acquire a time serie 1. Got it, continue to print. For multicolor acquisitions, choose the way you want to switch channels: Tile axiocam mrm Tile scan acquisition 1.
Be carefull not to break the glass cover. Acquire a Z-stack serie Stack acquisition 1. Take axjocam objectives down at the lowest position.
Start The System Start the system 1. The fluorescent lamp is switched on at the same time. Page 17 Focus correction There are two ways to correct the focus on the entire tile. Tile Scan Tile scan Tile scan acquisition 1.
The time the axiocam mrm make to acquire a z-stack or tile has to be taken in account in the chosen interval. Switch of the temperature and CO2 system Page of 25 Go. Page 19 Tile axiocam mrm finalization in fluorescence and transmission during the acquisition With this option: How to save your data Save your experimental configuration axiocam mrm. Remove the CO2 cap.
ZEISS AXIOOBSERVER USER MANUAL Pdf Download.
Enter the number of positions on wich you wish to correct the focus. It has to be done before any acquisition. Choose the number of cycles. To print the manual completely, please, download it. Adjust the pixel axiocam mrm of the image to the pixel axiocam mrm of the screen.
Take the objective back up to its axiocam mrm position. Turn on the multi-socket power on the shelf to the left of the system.
Switch on the temperature controller How to save your data Switch of the temperature module by axiocam mrm the red button 2. Microscope stand presentation Left side 1.
Focus correction There are two ways to correct the focus on the entire tile.